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COE > College of Education > Kinesiology > Research and Service > Centers and Labs > Research Labs > Exercise Vascular Cell Biology Lab

Centers and Labs - Exercise Vascular Cell Biology Lab

Image for Exercise Vascular Cell Biology Lab (EVCBL)

Research in the Exercise Vascular Cell Biology Lab examines exercise-induced signals for vascular adaptations, with emphasis on prevention and treatment of cardiovascular diseases. These “signals” include hemodynamic forces such as shear stress, as well as circulating factors such as endothelial progenitor/stem cells, inflammatory cytokines, and mediators of oxidative stress. Dr. Jenkins is the director of this laboratory. Our studies incorporate experimental tools and approaches from multiple disciplines including physiology, cellular biology, biochemistry, and functional genomics and involve both human subjects and rodent models of obesity and type 2 diabetes. Equipment includes a Bio-Rad CFX Connect Real Time qPCR system, a Class II Biosafety Cabinet and a humidified incubator for cell culture experiments, immunomagnetic cell separation magnets (EasySep), a fluorescent plate reader for studies of intracellular free radical dynamics, and general “wet lab” equipment for cellular/molecular biology work (pipettors, centrifuges, water baths, etc). Circulating levels of endothelial cell microparticles and endothelial progenitor cells are assessed via flow cytometric techniques using the instruments available in the UGA Flow Cytometry Core Facility.

Representative publications:

1.       Jenkins NT, Witkowski S, Spangenburg EE, Hagberg JM. Effects of acute and chronic endurance exercise on intracellular nitric oxide in putative endothelial progenitor cells: role of NADPH oxidase. Am J Physiol-Heart Circ Physiol 297(5): H1798-H1805, 2009.

2.       Witkowski S, Lockard MM, Jenkins NT, Obisesan TO, Spangenburg EE, Hagberg JM. Relationship between circulating progenitor cells, vascular function and oxidative stress with long-term training and short-term detraining in older men.Clin Sci (London) 118(4): 303-311, 2010.

3.       Jenkins NT, McKenzie JA, Damcott CL, Witkowski S, Hagberg JM.  Endurance exercise training effects on body fatness, VO2max, HDL-C subfractions, and glucose tolerance are influenced by a PLIN haplotype in older Caucasians. J Appl Physiol 108(3):498-506, 2010. *Received editorial focus: Ordovas JM and Smith CE. PLIN1 gene: fat keeper and prevention switcher.  J Appl Physiol 108(3): 477-478, 2010.

4.       Lockard MM, Witkowski S, Jenkins NT, Spangenburg EE, Obisesan TO, Hagberg JM.  Thrombin and exercise similarly influence expression of cell cycle genes in cultured putative endothelial progenitor cells. J Appl Physiol 108(6): 1682-1690, 2010.

5.       Jenkins NT, McKenzie JA, Hagberg JM, Witkowski S.  Plasma fetuin-A concentrations in young and older high- and low-active men. Metabolism 60(2): 265-271, 2011.

6.       Witkowski S, Jenkins NT, Hagberg JM. Enhancing treatment for cardiovascular disease: exercise and circulating angiogenic cells. Exerc Sport Sci Rev 39(2): 93-101, 2011.

7.       Jenkins NT, Landers RQ, Prior SJ, Soni N, Spangenburg EE, Hagberg JM. Effects of acute and chronic endurance exercise on intracellular nitric oxide and superoxide in circulating CD34+ and CD34- cells. J Appl Physiol 111(3): 929-937, 2011.

8.       Jenkins NT, Hagberg JM. Aerobic training effects on glucose tolerance in pre-diabetic and normoglycemic humans. Med Sci Sports Exerc43(12):2231-2240, 2011.

9.       Jenkins NT, Landers RQ, Thakkar SR, Fan X, Brown MD, Prior SJ, Spangenburg EE, Hagberg JM. Prior endurance exercise prevents postprandial lipemia-induced increases in reactive oxygen species in circulating CD31+ cells. J Physiol 589(22): 5539-5553, 2011.*Received editorial focus: Huffman DM, Calvert JW. Exercise to the Rescue. J Physiol, 589(24): 5919-5920, 2011.

10.   Bouchard C, Blair SN, Church TS, Earnest CP, Hagberg JM, Hakkinen K, Jenkins NT, Karavirta L, Kraus WE, Leon AS, Rao DC, Sarzynski MA, Skinner JS, Slentz CA, Rankinen T.  Adverse metabolic response to regular exercise: is it a rare or common occurrence? PLoS ONE 7(5): e37887, 2012.

11.   Jenkins NT, Martin JS, Laughlin MH, Padilla J. Exercise-induced signals for vascular endothelial adaptations: implications for cardiovascular disease. Curr Cardiovasc Risk Rep, 6(4):331-346, 2012.

12.   Martin JS, Padilla J, Jenkins NT, Crissey JM, Bender SB, Rector RS, Thyfault JP, Laughlin MH. Functional adaptations in the skeletal muscle microvasculature to endurance and interval sprint training in the type 2 diabetic OLETF rat. J Appl Physiol, 113(8):1223-1232, 2012.

13.   Jenkins NT, Padilla J, Arce-Esquivel AA, Bayless DS, Martin JS, Leidy HJ, Booth FW, Rector RS, Laughlin MH. Effects of endurance exercise training, metformin, and their combination on adipose tissue leptin and IL-10 secretion in OLETF rats. J Appl Physiol 113(12):1873-1883, 2012.

14.   Padilla J, Jenkins NT, Roberts MD, Arce-Esquivel AA, Martin JS, Laughlin MH, Booth FW. Differential changes in vascular mRNA levels between rat iliac and renal arteries produced by cessation of voluntary running. Exp Physiol 98(1):337-347, 2013.

15.   Jenkins NT, Padilla J, Boyle LJ, Credeur DP, Laughlin MH, Fadel PJ. Disturbed blood flow acutely induces activation and apoptosis of the human vascular endothelium. Hypertension 61(3): 615-621, 2013.

16.   Padilla J, Jenkins NT, Vieira-Potter VJ, Laughlin MH. Divergent phenotype of thoracic and abdominal perivascular adipose tissues. Am J Physiol-Reg Int Comp Physiol 304(7): R543-R552, 2013.

17.   Linden MA, Meers GM, Reubel ML, Jenkins NT, Booth FW, Laughlin MH, Ibdah JA, Thyfault JP, Rector RS. Hepatic steatosis development with four weeks of inactivity in previously active, hyperphagic OLETF rats. Am J Physiol-Reg Int Comp Physiol 304(9):R763-R771, 2013.

18.   Padilla J, Jenkins NT, Lee S, Zhang H, Cui J, Zuidema MY, Zhang C, Hill MA, Perfield JW II, Ibdah JA, Booth FW, Davis JW, Laughlin MH, Rector RS. Vascular transcriptional alterations produced by juvenile obesity in Ossabaw swine. Physiol Genomics 45(11):434-446, 2013.

19.   Jenkins NT, Padilla J, Rector RS, Laughlin MH. Physical activity increases β-adrenergic and natriuretic peptide receptor mRNAs in retroperitoneal adipose tissue of OLETF rats. Exp Physiol, in press [Epub Jul 5, 2013].

20.   Padilla J, Jenkins NT, Laughlin MH, Fadel PJ. Blood Pressure Regulation VIII: Resistance Vessel Tone and Implications for a Pro-Atherogenic Conduit Artery Endothelial Cell Phenotype. Invited Review. Eur J Appl Physiol, in press [Epub Jul 17, 2013].

21.   Crissey JM, Padilla J, Jenkins NT, Martin JS, Rector RS, Thyfault JP, Laughlin MH. Metformin does not enhance insulin-stimulated vasodilation in skeletal muscle resistance arteries of the OLETF rat. Microcirculation, in press [Epub Jul 24, 2013].

22.   Brandauer J, Landers-Ramos RQ, Jenkins NT, Spangenburg EE, Hagberg JM, Prior SJ. Effects of prior acute exercise on circulating cytokine concentration responses to a high-fat meal. Physiol Rep, in press. 

 

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